The purpose of this paper is to characterise aeration properties of 3 horizons of Arenic Chernozem profiles situated in Tišice (typical for the agricultural production region of Central Bohemia, Czech-Republic) on the basis of results obtained in the multilateral Austrian-Czech-Hungarian-Polish-Slovak project on the “Assessment of Structure in Agricultural Soils” sponsored by the Austrian Ministry of Science and Research. The paper comprises results of measurements of different soil aeration properties such as: oxygen diffusion rate (ODR), air-filled porosity (Eg), relative gas diffusion coefficient (D/Do), air permeability (k), and redox potential (Eh) as well as dehydrogenase and catalase activity. Undisturbed soil cores (from a depth of 5, 20 and 40 cm) were tested after equilibration on kaolin tension plates with soil water tensions 0 (capillary saturation – pF 0), 63 (pF 1.8), 159 (pF 2.2) and 500 hPa (pF 2.7). A significant correlation between the tested parameters was found.