The paper presents two methods (automatic and semi-automatic) for quantitative evaluation of cell structural parameters of plant tissues. The methods were developed for images obtained by confocal tandem scanning reflected light microscope. Quality of the images is sufficient for semi-automatic analysis. However, the automatic method does not give satisfactory results because it gives mean cell area 30% bigger and 60% fewer cells than in the semi-automatic method. Therefore, we state that for images taken by confocal tandem scanning reflected light microscope semi-automatic analysis is more accurate and proper at this moment.