The aim of the study was to investigate the effect of varying rates of FYM (0, 20, 40, 60 Mg ha^-1) and nitrogen N₀, N₁, N₂, and N₃ on the content of sulphate sulphur (VI) and the activity of arylsulphatase, which participates in the transformations of this element in Haplic Luvisol. The study report is based on a long-term field experiment with two different crop rotations: A – recognized as exhausting the humus from soil and B – recognized as enriching the soil with humus. During the cultivation of the plants, the soil was sampled four times from corn and a red clover cultivar and grass. The FYM fertilization rate for which the highest arylsulphatase activity and the content of sulphates were identified was 60 Mg ha^-1. An inhibitory effect of high rates (90 and 135 kg N ha^-1) of ammonium nitrate on the arylsulphatase activity was also observed. A significant correlation between the content of carbon, nitrogen, and sulphates and the arylsulphatase activity was recorded. The investigation on the effect of combined application of farmyard manure and mineral nitrogen fertilization on the activity of arylsulphatase participating in the sulphur cycling was launched to examine the problem in detail.