To investigate the effect of amended substrates on cadmium uptake by one-year old poplar rooted cuttings a pot culture was carried out. Pots were filled with three substrates. Four treatments of Cd supply including were organized. The results showed that higher biomass productions in substrates A and B compare to substrate C, led to an increase total Cd uptake two times more than that in substrate C, at 150 mg kg^-1 concentration. Meanwhile maximum total uptake occurred in substrate B at 100 mg kg^-1 concentration. Using synthetic chelators such as ethylenediaminetetraacetic acid in order to achieve high removal rate led to increased environmental impacts while they are not expected when such environmental friendly approaches are applied.