The present publication comprises results of measurements of aeration status related to soil properties such as: oxygen diffusion coefficient (D/Do), oxygen diffusion rate (ODR), redox potential (Eh), dehydrogenase and catalase activities as well as Fe+2 content at the soil moisture tensions of 63, 159 and 500 hPa in the (Ap) surface and (E) subsurface horizons of 3 soil profiles of an Orthic Luvisol under different land use (forest, farm and ex- perimental field). Results of the investigations showed differentiation in the aeration properties related to land use, soil horizons, water tension and reciprocal dependencies of aeration parameters.