RESEARCH PAPER
Correlation and path analysis of Tobacco (Nicotiana tabacum L.) yield vs root traits and relative water content as affected by Azotobacter, mycorrhizal symbiosis and biochar application under dry-land farming conditions
1
Department of Agronomy, North Tehran Branch, Islamic Azad University, Tehran, Iran
2
Department of Agronomy, Karaj Branch, Islamic Azad University, Karaj, Iran
3
Seed and Plant Improvement Institute (SPII), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
Final revision date: 2021-10-14
Acceptance date: 2021-11-15
Publication date: 2021-12-14
Corresponding author
Mohammad Reza Ardakani
Department of Agronomy, Karaj Branch, Islamic Azad University, Karaj, P.O. Box: 31499-6811, Iran
Department of Agronomy, Karaj Branch, Islamic Azad University, Karaj, P.O. Box: 31499-6811, Iran
Int. Agrophys. 2021, 35(4): 319-329
HIGHLIGHTS
- Tobacco production affected by water deficit due to climate alteration in semi-arid regions.
- Biochar improves water holding capacity in soil.
- Phytochemical components affected by interactions between mycorrhiza and Azotobacter.
- 4 ton/ha biochar with mycorrhiza alleviate the negative effects of water deficit.
- Azotobacter significantly increased root length and nicotine content.
KEYWORDS
TOPICS
ABSTRACT
The global approach in agriculture is to reduce the use of chemical fertilizers and the supply of nutrients from available sources which are environmentally friendly. In order to evaluate the feasibility of tobacco products without chemical fertilizer inputs, this research was carried out as a factorial experiment based on a 3×2×2 randomized complete block design which included biochar applied at three levels (0, 4, and 8 t ha-1), mycorrhiza, and Azotobacter at two levels (with and without application) with four replications. According to the results, 4 t ha-1 biochar increased the dry yield by 22%, the relative water content by 6%, and the root length by 41% compared to the zero level. However, there was no statistically significant difference between the 4 and 8 t ha-1 application of biochar with regard to most traits. The application of mycorrhiza improved the leaf area index as well as the tobacco root length. Azotobacter significantly increased the root length and nicotine content. The tobacco yield in rain-fed conditions is lower than usual, therefore the combined use of biochar and these biofertilizers may be considered as a viable solution. With increasing interest in the use of environmentally friendly sources of fertilizers and in terms of economic considerations, the use of 4 t ha-1 of biochar along with mycorrhiza and Azotobacter achieved an acceptable yield.
REFERENCES (50)
1.
Abbas T., Rizwan M., Ali S., Rehman M.Z., Qayyum M.F., Abbas F., Hannan F., Rinklebe J., and Ok Y.S., 2017. Effect of biochar on cadmium bioavailability and uptake in wheat (Triticum aestivum L.) grown in soil with aged contamination. Ecotoxicol Environ. Saf., 140, 37-47. https://doi.org/10.1016/j.ecoe....
2.
Ahanger M.A., Tyagi S.R., Wani M.R., and Ahmad P., 2014. Drought Tolerance: Role of organic osmolytes, growth regulators, and mineral nutrients. In: Physiological Mechanisms and Adaptation Strategies in Plants Under Changing Environment (Eds P. Ahmad, M.R. Wani). Springer, New York, USA. https://doi.org/10.1007/978-1-....
3.
Alarcón A., Davies Jr F.T., Egilla J.N., Fox T.C., Estrada-Luna A., and Ferrera-Cerrato R., 2002. Short term effects of Glomus claroideum and Azospirillum brasilense on growth and root acid phosphatase activity of Carica papaya L. under phosphorus stress. Rev. Latinoam. Microbiol., 44(1), 31-37.
4.
Antony A. and Singadhupe R.B., 2004. Impact of drip and surface irrigation on growth, yield and WUE of capsicum (Capsicum annum L.). Agric. Water Manag., 65(2), 121-132. https://doi:10.1016/j.agwat.20....
5.
Ardakani M.R., Pietsch G., Moghaddam A., Raza A., and Friedel J.K., 2009. Response of root properties to tripartite symbiosis between lucerne (Medicago sativa L.), rhizobia and mycorrhiza under dry organic farming conditions. Am. J. Agric. Biol. Sci., 4, 266-277. https://doi:10.3844/ajabssp.20....
6.
Azcon R. and Barea J.M., 1976. Synthesis of auxins, gibberellins and cytokinins by Azotobacter vinelandii and Azotobacter beijerinckii related to effects produced on tomato plants. Plant Soil, 43, 609-613. https://doi.org/10.1007/BF0192....
7.
Azimi R., Gang Ju M., and Asgari H.R., 2013. The effect of mycorrhiza inoculation on the initial establishment and morphological traits of the Thymus vulgaris under natural conditions (in Persian). Iranian J. Field Crop Res., 11(4), 666-676. https://doi.org/10.22067/GSC.V....
8.
Baldwin I.T., 2001. An ecologically motivated analysis of plant herbivore interactions in native tobacco. Plant Physiol., 127, 1449-1458. https://doi.org/10.1104/pp.010....
9.
Bashan Y. and de-Bashan L.E., 2005. Plant Growth-Promoting. Encyclopedia. Soils Environ., 1, 103-115. https://doi.org/10.1016/B0-12-....
10.
Baum C., El-Tohamy W., and Gruda N., 2015. Increasing the productivity and product quality of vegetable crops using arbuscular mycorrhizal fungi: a review. Sci Hort., 87, 131-141. https://doi.org/10.1016/j.scie....
11.
Behrooz A., Vahdati K., Rejali F., Lotfi M., Sarikhani S., and Lesile C., 2019. Arbuscular mycorrhiza and plant growth-promoting bacteria alleviate drought stress in walnut. Hort. Science, 54(6), 1087-1092. https://doi.org/10.21273/HORTS....
12.
CORESTA, 1994. CORESTA recommended method No 35. Determination of total alkaloids (as nicotine) in tobacco by continuous flow analysis. https://www.coresta.org/sites/... (Aug10) pdf.
13.
Dai A., 2012. Drought under global warming: a review. Wires Clim Chg., 2, 45-65. https://doi.org/10.1002/wcc.81.
14.
Downie A., Crosky A., and Munroe P., 2009. Physical properties of biochar. In: Biochar for Environmental Management: Science and Technology (Eds J. Lehmann, S. Joseph). London, Earthscan.
15.
Dixon R.K., Rao M.V., and Garg V.K., 1993. water relations and gas exchange of mycorrhizal Leucaena leucocephala seedlings. Trop. For. Sci., 6, 542-552.
16.
El-Sawah AM., El-Keblawy A., Ali D.F.I., Ibrahim H.M., El-Sheikh M.A., Sharma A., Alhaj Hamoud Y., Shaghaleh H., Brestic M., Skalicky M., Skalicky M., Xiong Y.C., and Sheteiwy M., 2021. Arbuscular mycorrhizal fungi and plant growth-promoting rhizobacteria enhance soil key enzymes, plant growth, seed yield, and qualitative attributes of guar. Agriculture, 11(3), 194. https://doi.org/10.3390/agricu....
17.
Gao C., El-Sawah AM., Fathi Ismail Ali D., Alhaj Hamoud Y., Shaghaleh H., and Sheteiwy M.S., 2020. The integration of bio and organic fertilizers improve plant growth, grain yield, quality and metabolism of hybrid maize (Zea mays L.). Agronomy, 10(3), 319. https://doi.org/10.3390/agrono....
18.
Gebhardt M., Fehmi J.S., Rasmussen C., and Gallery R.E., 2017. Soil amendments alter plant biomass and soil microbial activity in a semi-desert grassland. Plant Soil, 419, 53-70. https://doi.org/10.1007/s11104....
19.
Giovannetti M. and Mosse B., 1980. An evaluation of techniques for measuring vesicular arbuscular mycorrhizal infection in roots. New Phytol., 84(3), 489-500. https://doi:10.1111/j.1469-813....
20.
Gul S. and Whalen J.K., 2016. Biochemical cycling of nitrogen and phosphorus cycling in biochar amended soils. Soil Biol. Biochem., 103, 1-15. https://doi.org/10.1016/j.soil....
21.
Hammer E.C., Forstreuter M., Rillig M.C., and Kohler J., 2015. Biochar increases arbuscular mycorrhizal plant growth enhancement and ameliorates salinity stress. Appl. Soil Ecol., 96, 114-121. https://doi.org/10.1016/j.apso....
22.
Hashem A., Kumar A., Al-Dbass A.M., Alqarawi A.A., Al-Arjani A.B.F., Singh G., Farooq M., and Abd-Allah E.F., 2019. Arbuscular mycorrhizal fungi and biochar improves drought tolerance in chickpea. Saudi J. Biol. Sci., 26, 614-624. https://doi.org/10.1016/j.sjbs....
23.
Ilkaee M.N., Paknejad F., Zavareh M., Ardakani M.R., and Kashani A., 2011. Prediction model of leaf area in soybean (Glycine max L.). Am. J. Agric. Biol. Sci., 6, 110-113. https://doi.org/10.3844/ajabss....
24.
Ju X.T., Chao FC., Li C.J., Jiang R.F., Christie P., and Zhang F.S., 2008. Yield and nicotine content of flue-cured tobacco as affected by soil nitrogen mineralization. Pedosphere, 18(2), 227-235. https://doi.org/10.1016/S1002-....
25.
Kim Y., Oh J.-I., Vithanage M., Park Y.-K., Lee J., and Kwon E.E., 2019. Modification of biochar properties using CO2. Chemical Eng. J., 372, 383-389. https://doi.org/10.1016/j.cej.....
26.
Laird D.A., 2008. The charcoal vision: a win-win-win scenario for simultaneously producing bioenergy, permanently sequestering carbon, while improving soil and water quality. Agron. J., 100, 178-181. https://doi.org/10.2134/agronj....
27.
Larsen J., Pineda-Sánchez H., Delgado-Arellano I., Castellano-Morales V., Carreto-Montoya L., and Villegas-Moreno J., 2017. Interactions between microbial plant growth promoters and their effects on maize growth performance in different mineral and organic fertilization scenarios. Rhizosphere, 3, 75-81. https://doi.org/10.1016/j.rhis....
28.
Major J., Rondon M., Molina D., Riha SJ., and Lehmann J., 2010. Maize yield and nutrition during 4 years after biochar application to a Colombian savanna oxisol. Plant Soil, 333, 117-128. https://doi.org/10.1007/s11104....
29.
Mardukhi B., Rejali F., Daei G., Ardakani M.R., Malakouti M.J., and Miransari M., 2015. Mineral uptake of mycorrhizal wheat (Triticum aestivum L.) under salinity stress. Commun. Soil. Sci. Plant Analysis, 46, 343-357. https://doi.org/10.1080/001036....
30.
Mehari Z.H., Elad Y., Rav-David D., Graber E.R., and Harel Y.M., 2015. Induced systemic resistance in tomato (Solanum lycopersicum) against Botrytis cinerea by biochar amendment involves jasmonic acid signaling. Plant Soil, 395, 31-44. https://doi.org/10.1007/s11104....
31.
Mickan B., 2014. Mechanisms for alleviating of water stress involving arbuscular mycorrhizal fungi. In: Mycorrhizal Fungi: Use in Sustainable Agriculture and Land Restoration (Eds Z.M. Solaiman, L.K. Abbott, A. Varma). Soil Biol., 41, 225-239. https://doi:10.1007/978-3-662-....
32.
Miri M.R., Tohidi Moghadam H.R., Ghooshchi F., and Zahedi H., 2013. Effect of Azotobacter and Arbuscular mycorrhizal colonization enhance wheat growth and physiological traits under well-watered and drought conditions. J. Advances Environ. Biol., 7(14), 4630-4636.
33.
Moosavi SA., Shokuhfar A., Lak S., Mogaddam M., and Alavifazel M., 2020. Integrated application of biochar and bio-fertilizer improves yield and yield components of cowpea under water-deficient stress. Ital. J. Agron., 15, 94-101. https://doi.org/10.4081/ija.20....
34.
Oppong Danso E., Yakubu A., Arthur E., Sabi E.B., Abenney-Mickson S., and Andersen M.N., 2020. Effect of rice straw biochar and irrigation on growth, dry matter yield and radiation-use efficiency of maize grown on an Acrisol in Ghana. J. Agron. Crop Sci., 206, 296-307. https://doi.org/10.1111/jac.12....
35.
Pasban Eslam B., Monirifar H., and Sadeghi Bakhtavari A.R., 2017. Morpho-physiological response of rapeseed (Brassica napus L.) genotypes to drought stress. Crop Breed. J., 7, 49-56. https://doi.org/10.22092/cbj.2....
36.
Phillips J.M. and Hayman D.S., 1970. Improved procedures for clearing roots and staining parasitic and vesicular arbuscular mycorrhizal fungi for rapid assessment of infection. Trans. British Mycolog. Society, 55, 158-161.
37.
Samarbakhsh S., Rejali F., Ardakani M.R., Nejad F.P., and Miransari M., 2009. The combined effects of fungicides and arbuscular mycorrhiza on corn (Zea mays L.) growth and yield under field conditions. J. Biol. Sci., 9, 372-376. https://doi.org/10.3923/jbs.20....
38.
Saribay G.F., 2003. Growth and nitrogen fixation dynamics of Azotobacter chroococcum nitrogen-free and OMW containing medium. M.Sc. Thesis, The Graduate School of Natural and Applied Sciences, Middle East Technical University, Turkey.
39.
Shang X., Shang Y., Fu J., and Zhan T., 2017. Nicotine significantly improves chronic stress-induced impairments of cognition and synaptic plasticity in mice. Molecular Neurobiol., 54, 4644-4658. https://doi.org/10.1007/s12035....
40.
Sharma A.K., 2002. Biofertilizers for Sustainable Agriculture. Agro-Bios, Jodhpur India.
41.
Sheteiwy M.S., Ali D.F.I., Xiong Y.C., Brestic M., Skalicky M., Alhaj Hamoud Y., Ulhassan Z., Shaghaleh H., AbdElgawad H., Farooq M., Sharma A., and El-Sawah A.M., 2021. Physiological and biochemical responses of soybean plants inoculated with Arbuscular mycorrhizal fungi and Bradyrhizobium under drought stress. BMC Plant Biol., 21, 195. https://doi.org/10.1186/s12870....
42.
Smart R. and Bingham G., 1974. Rapid estimates of relative water content. Plant Physiol., 53, 258-260.
43.
Soliman M.H., Abdulmajeed A.M., Alhaithloul H., Alharbi B.M., El-Esawi M.A., Hasanuzzaman M., and Elkelish A., 2020. Saponin biopriming positively stimulates antioxidants defense, osmolytes metabolism and ionic status to confer salt stress tolerance in soybean. Acta Physiol. Plant, 42, 114. https://doi.org/10.1007/s11738....
44.
Suliman W., Harsh J.B., Abu-Lail N.I., Fortun A.M., Dallmeyer I., and Garcia Pérez M., 2017. The role of biochar porosity and surface functionality in augmenting hydrologic properties of a sandy soil. Sci. Total Environ., 574, 139-147. https://doi.org/10.1016/j.scit....
45.
Syamsiyah J. and Herawati A., 2018. The potential of arbuscular mycorrhizal fungi application on aggregrate stability in alfisol soil. In Proceedings of the IOP Conference Series: Earth Environ. Sci., Surakarta, Indonesia, 142, 012045. https://doi.org/10.1088/1755-1....
46.
Tarnabi Z.M., Iranbakhsh A., Mehregan I., and Ahmadvand R., 2019. Impact of arbuscular mycorrhizal fungi (AMF) on gene expression of some cell wall and membrane elements of wheat (Triticum aestivum L.) under water deficit using transcriptome analysis. Physiol. Mol. Biol. Plant, 26(1), 143-162. https://doi.org/10.1007/s12298....
47.
Tso TC., 1990. Production, Physiology, and Biochemistry of Tobacco Plant. Ideals Inc., Beltsville, ML, USA.
48.
Van der Heijden M.G., Martin F.M., Selosse M.A., and Sanders I.R., 2015. Mycorrhizal ecology and evolution: the past, the present, and the future. New Phytol., 205, 1406-1423. https://doi.org/10.1111/nph.13....
49.
Wu Q.S. and Zou Y.N., 2009. Mycorrhiza has a direct effect on reactive oxygen metabolism of drought-stressed citrus. Plant Soil Environ., 55, 436-442. https://doi.org/10.17221/61/20....
50.
Xiang Y., Deng Q., Duan H., and Guo Y., 2017. Effects of biochar application on root traits. GCB Bioenergy, 9, 1563-1572. https://doi.org/10.1111/gcbb.1....
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