RESEARCH PAPER
Early detection of root-knot nematode (Meloidogyne incognita) infection by monitoring root dielectric response non-destructively
1
Department of Zoology and Ecology, Hungarian University of Agriculture and Life Sciences, Páter Károly u. 1.,
H-2100 Gödöllő, Hungary
2
Department of Soil Physics and Water Management, Institute for Soil Sciences, Centre for Agricultural Research, ELKH,
Herman Ottó út 15., H-1022 Budapest, Hungary
Final revision date: 2023-03-24
Acceptance date: 2023-03-29
Publication date: 2023-05-17
Corresponding author
Imre Cseresnyés
Department of Soil Physics and Water Management, Institute for Soil Sciences, Centre for Agricultural Research, ELKH, Herman Ottó út 15., H-1022, Budapest, Hungary
Department of Soil Physics and Water Management, Institute for Soil Sciences, Centre for Agricultural Research, ELKH, Herman Ottó út 15., H-1022, Budapest, Hungary
Int. Agrophys. 2023, 37(2): 179-187
HIGHLIGHTS
- We first used root dielectric measurement to detect Meloidogyne infestation in situ.
- Nematode entry and development markedly changed the root dielectric properties.
- The effect was due to altered root anatomy and physiology linked to gall formation.
- Obvious aboveground symptoms did not appeared on infected plants.
- The presented non-destructive method can contribute to an improved nematode control.
KEYWORDS
chlorophyllelectrical capacitanceelectrical conductancein situ root methodsplant-parasitic nematode detectionsoil-borne pest
TOPICS
ABSTRACT
The early recognition of root-knot nematode injury belowground is essential in order to avoid serious crop losses. The measurement efficiency of the root dielectric response for detecting Meloidogyne incognita infection non-destructively was tested in potted cucumber and tomato. The electrical capacitance, dissipation factor and electrical conductance of the root, and also the leaf chlorophyll concentration were measured instrumentally three times during plant growth, this was followed by an evaluation of the root galling intensity after harvest. The electrical capacitance and conductance increased significantly shortly after Meloidogyne infection, this was likely due to the substantially enhanced surface area and electrolyte permeability of the root membranes during giant cell formation. The dissipation factor and electrical conductance (related to hydraulic conductance) markedly decreased at the late stage of nematode infection, this was due to restricted root growth and solute uptake caused by the intrusion of giant cells into the root vascular tissues. No serious aboveground pest symptoms were visible in the plants studied owing to the low inoculum density. The results demonstrated the potential of dielectric measurement for the early detection of root-knot nematode infection without plant damage, before the appearance of obvious disease symptoms. This diagnostic tool has the potential to contribute to the improved selection of Meloidogyne-resistant crop genotypes, as well as more efficient nematode control to mitigate economic losses.
ACKNOWLEDGEMENTS
The authors thank Barbara Harasztos for language editing and anonymous reviewers for their valuable remarks.
FUNDING
The work was funded by the National Research, Development and Innovation Fund of Hungary (NKFIH), project No. 137617, financed under the FK-21 funding scheme (2021-2025).
CONFLICT OF INTEREST
The authors declare that they have no conflict of interest.
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