The effect of variations in NPK fertilization on dehydrogenase and catalase activity of rhizosphere and non-rhizosphere of two varieties of amaranth – Rawa and Aztek was determined. In a field experiment, amaranth was grown at wide row spacing on good wheat complex soil in SE Poland. Dehydrogenase and catalase activity in the rhizosphere and non-rhizosphere were determined. The results showed that fertilization with increasing doses of NPK influenced enzyme activity in the rhizosphere and non-rhizosphere of two varieties of amaranth. Fertilization with the highest dose of the macroelements had the greatest effect. Enzyme activity in the rhizosphere of the plants studied was higher than in the non-rhizosphere soil in the case of both amaranth varieties. Significant positive correlations were observed between the NPK doses applied and activity of the enzymes, in the rhizosphere and non-rhizosphere of both amaranth varieties.