The paper demonstrates the results of research on the mobility of phosphorus and sulphur in winter wheat fertilized with several rates (0, 20, 40, 60, 80 t ha^-1) of farmyard manure and nitrogen (0, 40, 80, 120 kg N ha^-1). The content of these nutrients was related to the activity of acid phosphatase and arylsulphatase in a Haplic Luvisol. The highest content of available phosphorus (91.58 mg P kg^-1) was reported in the soil amended with farmyard manure at the rate of 60 t ha^-1. The content of sulphates (VI) in the Haplic Luvisol was high and, on average, equal to 25.22 mg kg^-1. The activity of acid phosphatase in the soil increased with increasing mineral nitrogen rates. The highest content of sulphates (VI) and the lowest activity of arylsulphatase were identified at the nitrogen rate of 40 kg N ha^-1. The mobility of phosphorus in winter wheat was the highest when farmyard manure at the rate of 60 t ha^-1 and mineral nitrogen at the rate of 120 kg N ha^-1 were incorporated into the soil. The greatest translocation of sulphur was reported at the high farmyard manure rates (40, 60 and 80 t ha^-1) and the nitrogen rate of 80 kg N ha^-1.