Institute of Botany, University of the Punjab, Lahore Pakistan
2
Department of Food Science and Nutrition, College of Agriculture Food Science, King Saud University, Riyadh, Saudi Arabia
3
Department of Pharmacology, Institute of Pharmacy, Faculty of Pharmaceutical and Allied Health Sciences, Lahore College for Women University, Lahore, Pakistan
4
Department of Food Science and Technology, Faculty of Food and Home Sciences, MNS-University of Agriculture Multan, Pakistan
5
Department of Soil and Environment, Faculty of Crop Protection, Sindh Agriculture University, Tandojam, Pakistan
6
Department of Botany and Microbiology, College of Science, Saudi Arabia
7
Institute of Agrophysics, Polish Academy of Sciences, Doświadczalna 4, 20-290 Lublin, Poland
8
Faculty of Production and Power Engineering, University of Agriculture in Kraków, Balicka 116B, 30-149 Kraków, Poland
9
Center of Innovation and Research on Healthy and Safe Food, University of Agriculture in Kraków, Balicka 104, 30-149 Kraków, Poland
Final revision date: 2024-10-21
Acceptance date: 2024-11-21
Publication date: 2025-02-24
Corresponding author
Mateusz Stasiak
Institute of Agrophysics, Polish Academy of Sciences, Doświadczalna 4, 20-290, Lublin, Poland
Beans and lentils are good sources of overall phenolic content and have high levels of free radical scavenging capacity. The goal of the present study was to evaluate the phenolic and antioxidant content of both dried and germinated beans and lentils. In order to do this, total phenolic content, 2-2-diphenyl-1-picrylhydrazine, and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) antioxidant were measured using the Folin-Ciocalteu method. Seven different varieties of beans and lentils were used to extract total phenolic content using 50% (v/v) aqueous ethanol. In samples of germinated seeds, the legumes showed significantly (p<0.05%) higher total phenolic content, 2-2-diphenyl-1-picrylhydrazine, and antioxidant activity. However, the analysis revealed an increase in the total phenolic content content in sprouted beans and lentils and decreased activity in dried beans. The present study suggests that the content of phenolics and antioxidant
activity in legumes are increased during the germination process, but are lower in dried samples. The present work supports the idea that germinated beans are a natural source of antioxidants that can be used commercially.
FUNDING
This work was funded by the Researchers Supporting Project, number (RSPD2025R637), King Saud University, Riyadh, Saudi Arabia.
CONFLICT OF INTEREST
The Authors do not declare any conflict of interest.
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