A pot experiment with triticale cv. Jago was conducted to test the effect of oxygen deficiency on the content of available macroelements. Oxygen stress (flooded conditions) distinctly conserved all the chemical species content but only at lowest density of soil combined with flooded conditions the decline of uptake of N-NH4+ and P and K was significant. A significant increase in (p <0.001) uptake of the investigated elements occurred in higher values of air filled porosity (Eg). The most sensitive element with respect to Eg value changes was phosphorus, the least one was potassium. The relation between redox potential (Eh) on NH4+, NO3–, P and K uptake was positive in all the investigated cases. The highest relation was observed in P uptake. The parameter of oxygen availability in the soil – ODR (oxygen diffusion rate) showed a typical tendency for changes under differentiated air-water conditions. The critical ODR value (about 35 g m-2 s-1) for uptake of all the studied elements was observed under the investigated conditions. Statistical analysis showed a significant relation between oxygen diffusion rate and NH4+, P and K uptake.