The purpose of the conducted researches was an estimation of influencing of application of growth regulators (metconazole, di-1-P-menten, dimethipin) and desiccants (diquat, glyphosate) on a yield and quality of mass of 1000 seeds (MTS). Influencing of fertilization method of rape is taken into account at the same time. The obtained results specify that the delay of harvest of rape (term I – full maturity, term II – 10 days after) has a substantial influence on the yield of seeds. Losses from that delay can account for as much as 10.9 q ha-1. Application of Caramba (especially in the variant of B and C) had a substantial influence on the yield growth (about 5-6 q ha-1) at collections in the term I, and even stronger in the term II (6-7 q ha-1). The applied regulators of ripening (especially Reglone and Roundup) had a negative influence on the yield growth, causing its decrease of about 2-3 q ha-1 (Reglone alone applied in the II term of harvest had a very slight impact on the yield growth). Applied chemicals modified MTS only in an insignificant way (statistically unimportant). Substantial differences were found only between control and combination where Roundup was applied – but only in the term II of harvest. The meaningful decrease of MTS was also found on the combination where Reglone was applied. However, they were statistically insignificant.