The aim of the study was to determine under laboratory conditions the respiration rate of carbon rock spoil, after different time of storage, amended with municipal wastewater after mechanical and biological treatment in relation to pH, redox potential and temperature. The experiment was carried out on the coal mine rocks exposed to weathering for five and ten years under atmospheric conditions. Respiration rates show growing tendency with increase of temperature. Respiration rate of the 5-year material was higher than that of the 10-year material. The respiration values after addition of wastewater were 1.30 and 2.72 times greater than after addition of distilled water for the 5- and 10-year, respectively. Flux of carbon dioxide showed positive linear correlation with pH and negative linear correlation with Eh values.